Standard Operating Procedure for Ender 3S1 Bioprinter --------------------------------------------------------- 1. Turn on Ender 3S1 power 2. Slide CRTOUCH probe out of storage bracket and into hotend bracket 3. Select Prepare -> Homing -> Auto Home 4. Remove CRTOUCH probe from hotend bracket and stow away again 4. Select Prepare -> Preheat PLA 5. Once bed and extruder temperatures have reached setpoints, set HV level and extrude rate: a) Run Applications -> System -> CuteCom b) Open device /dev/ttyACM1 c) be sure DTR is checked d) type in Input field then enter: {"hvpos", 0} (sets polarity to negative - yel LED comes on)) {"hvlev", 4000} (sets high voltage level to 4kv) {"hvon", 1} (turns on HV - red LED comes on) {"rate", 10} (extrude rate = 10uL/min) 6. Select Media -> program name This runs the G-code you have prepared with Cura MEW profile