Using the 2GBP ---------------------- 1. open a terminal window 2. cd linuxcnc/configs/2gbp11a 3. run: linuxcnc 2gbp11a.ini 4. turn on box power 5. home all axes 6. use syringe adjust retract/depress buttons to align extruder 7. load g-code file with File->Open or the file folder icon 8. if electrospinning, set syringe spinning rate and press HV Armed 9. press the -> (play) button to run g-code program NOTE - IF HV VOLTAGE READS OVER 0.8, HV IS PRESENT! Programming ----------------------- Commands and parameters: M68 E0 Q0,1, or 2 0=idle 1=mL/mm 2=mL/hr M68 E1 Qxx Extrude rate, either mL/mm or mL/hr M68 E2 Q0 or 1 0=HV off, 1=HV on if armed (eventually 0 or hv val) There are four modes of operation: 1. bioprinting: o call xx - extrusion in mL/mm rate M68 E0 Q1 M68 E1 Qxx - no hv M68 E2 Q0 2. electroprinting or nfes: o call xx vv - extrusion in mL/mm M68 E0 Q1 M68 E1 Qxx - hv enabled M68 E2 Q1 3. electrospinning: o call xx vv - extrusion in mL/hr M68 E0 Q2 M68 E1 Qxx - hv enabled M68 E2 Q1 4. no-print motion: o call - no extrusion M68 E0 Q0 - no hv M68 E2 Q0 To extrude (3d print) at 0.1mL/mm rate: o call 0.1 To electroprint at 0.1mL/mm at 10kv: o call 0.1 10000 To electrospin at 0.01mL/hr rate at 10kv for 10min: o call 0.01 10000 G4 P600 To stop extruding or electrospinning: o call